Characterization of the Metallothioneins Induced in HeLa Cells by Dexamethasone and Zinc
Open Access
- 1 June 1980
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 107 (2), 395-401
- https://doi.org/10.1111/j.1432-1033.1980.tb06042.x
Abstract
The metallothioneins induced in HeLa cells by dexamethasone and Zn2+ were labeled with [3H]cysteine and [35S]cysteine respectively. These labeled metallothioneins were compared to one another and to metallothioneins from human liver by (a) gel permeation chromatography on Sephadex G-75 columns, (b) ion-exchange chromatography on DEAE-Sephadex A-25 columns, (c) electrophoresis on non-denaturing polyacrylamide gels, and (d) electrophoresis of reduced and alkylated proteins on dodecylsulfate/polyacrylamide gels. The dexamethasone-induced metallothioneins of HeLa cells, the Zn2+-induced metallothioneins of HeLa cells, and the metallothioneins of human liver were indistinguishable by these four criteria.This publication has 32 references indexed in Scilit:
- Primary induction of metallothionein by dexamethasone in cultured rat hepatocytesBiochemical and Biophysical Research Communications, 1980
- Regulation of liver metallothionein and plasma zinc by the glucocorticoid dexamethasoneBiochemical and Biophysical Research Communications, 1979
- Synthesis of metallothionein in a polysomal cell-free systemBiochemical and Biophysical Research Communications, 1977
- Distribution of cadmium, zinc and copper in the musselMytilus edulis. Existence of cadmium-binding proteins similar to metallothioneinsCellular and Molecular Life Sciences, 1976
- Isolation and purification of cadmium binding proteins from rat liverBiochemical and Biophysical Research Communications, 1974
- Human hepatic metallothioneinsFEBS Letters, 1974
- The regulation of 4 forms of tyrosine aminotransferase in adult and developing rat liverLife Sciences, 1971
- Fractionation of rat liver tyrosine aminotransferase during the course of purification. Further evidence for multiple forms of the enzymeFEBS Letters, 1970
- Multiple forms of tyrosine aminotransferase in rat liver and their hormonal induction in the neonateFEBS Letters, 1969
- A CADMIUM PROTEIN FROM EQUINE KIDNEY CORTEXJournal of the American Chemical Society, 1957