Continuous direct photometry of dyed materials in filter paper with special reference to the estimation of proteins separated by electrophoresis

Abstract

An apparatus is descr. for continuous direct photometry of dyed materials in filter paper, which has been successfully used for estimation of proteins separated by electrophoresis. The applicability of Beer''s law to the absorption of light by dyed protein in an immersed filter paper was tested experimentally. It was found that, outside of a very restricted range, large deviations occur. A method of calibration is descr. by which the deviation from Beer''s law may be estimated and compensated for. The relation between the results of electrophoretic analysis of proteins in paper and in free solution are discussed.