Novel Techniques for Weak Alignment of Proteins in Solution Using Chemical Tags Coordinating Lanthanide Ions
- 1 July 2004
- journal article
- Published by Springer Nature in Journal of Biomolecular NMR
- Vol. 29 (3), 339-349
- https://doi.org/10.1023/b:jnmr.0000032611.72827.de
Abstract
A molecule with an anisotropic magnetic susceptibility is spontaneously aligned in a static magnetic field. Alignment of such a molecule yields residual dipolar couplings and pseudocontact shifts. Lanthanide ions have recently been successfully used to provide an anisotropic magnetic susceptibility in target molecules either by replacing a calcium ion with a lanthanide ion in calcium-binding proteins or by attaching an EDTA derivative to a cysteine residue via a disulfide bond. Here we describe a novel enantiomerically pure EDTA derived tag that aligns stronger due to its shorter linker and does not suffer from stereochemical diversity upon lanthanide complexation. We observed residual 15N,1H-dipolar couplings of up to 8 Hz at 800 MHz induced by a single alignment tensor from this tag.Keywords
This publication has 28 references indexed in Scilit:
- De Novo Determination of Bond Orientations and Order Parameters from Residual Dipolar Couplings with High AccuracyJournal of the American Chemical Society, 2003
- Dipolar Couplings in Multiple Alignments Suggest α Helical Motion in UbiquitinJournal of the American Chemical Society, 2003
- NMR Solution Structure and Dynamics of the Peptidyl-prolyl cis–trans Isomerase Domain of the Trigger Factor from Mycoplasma genitalium Compared to FK506-binding ProteinJournal of Molecular Biology, 2002
- Structural Characterization of Proteins with an Attached ATCUN Motif by Paramagnetic Relaxation Enhancement NMR SpectroscopyJournal of the American Chemical Society, 2001
- Paramagnetism-based versus classical constraints: An analysis of the solution structure of Ca Ln calbindin D9kJournal of Biomolecular NMR, 2001
- Calmodulin tagging provides a general method of using lanthanide induced magnetic field orientation to observe residual dipolar couplings in proteins in solutionJournal of Biomolecular NMR, 2001
- Protein–protein interactions mapped by artificial proteases: where σ factors bind to RNA polymeraseTrends in Biochemical Sciences, 2000
- Determination of Internuclear Angles of DNA Using Paramagnetic-Assisted Magnetic AlignmentJournal of Magnetic Resonance, 1998
- Nuclear magnetic dipole interactions in field-oriented proteins: information for structure determination in solution.Proceedings of the National Academy of Sciences, 1995
- Mixed thiolsulfonates and sulfonamides from polyfunctional mercaptans using trifluoromethyl thiosulfonatesJournal of Medicinal Chemistry, 1972