Nutritional and hormonal requirements of Ginkgo biloba embryo-derived callus and suspension cell culture
- 1 April 1990
- journal article
- research article
- Published by Springer Nature in Plant Cell Reports
- Vol. 8 (11), 635-638
- https://doi.org/10.1007/bf00269981
Abstract
Calli were obtained from Ginkgo biloba embryos grown on Murashige and Skoog (MS) medium. The G. biloba cells could grow on either MS or Gamborg B5 mineral salt medium supplemented with sucrose (3% and 2%, respectively) and naphthaleneacetic acid (NAA) and kinetin (K) in concentrations ranging from 0.1 to 2.0 mg·L−1. Best growth and maintenance of callus cultures were achieved using MS medium supplemented with 2 mg·L−1 NAA and 1 mg·L−1 K (N2K1MS). Light was required to maintain healthy growth of the callus tissue. In both MS and B5 based media, sucrose was hydrolyzed extracellularly before being taken up by Ginkgo cell suspension cultures. Specific growth rates of 0.13 d−1 and 0.08 d−1 were obtained in MS medium supplemented with 1 mg·L−1 NAA, 0.1 mg·L−1 K and 30 g·L−1 sucrose (N1K0.1MS) and B5 medium supplemented with the same growth regulator regime and 20 g·L−1 sucrose (N1K0.1B5) respectively. Complete phosphate and ammonium uptake was observed in 11 days when cultured in MS medium and 10 days and 4 days respectively when cultured in B5 medium. During the culture, G. biloba cells consumed only 64% and 29% of the nitrate content of N1K0.1MS and N1K0.1B5 media respectively. Maximum dry biomass concentrations were 13.4 g·L−1 and 7.9 g·L−1, and yields on carbohydrate were 0.39 and 0.45 in N1K0.1MS and N1K0.1B5 media respectively. The better performance of MS cultures came from the higher sucrose and nitrogen salts concentrations of this medium.Keywords
This publication has 9 references indexed in Scilit:
- Ammonium Triggers Uptake of NO−3 by Chenopodium rubrum Suspension Culture Cells and Remobilization of their Vacuolar Nitrate PoolPlant and Cell Physiology, 1989
- Preparation and Definition of Ginkgo Biloba ExtractPublished by Springer Nature ,1988
- Nutrient requirements of suspension cultures of soybean root cellsExperimental Cell Research, 1968
- Plastid Function in Plant Tissue Cultures. I. Porphyrin Synthesis by Dark-Grown Haploid and Diploid Albino CulturesAmerican Journal of Botany, 1967
- PLASTID FUNCTION IN PLANT TISSUE CULTURES. I. PORPHYRIN SYNTHESIS BY DARK‐GROWN HAPLOID AND DIPLOID ALBINO CULTURESAmerican Journal of Botany, 1967
- A Haploid Tissue Culture from the Female Gametophyte of Ginkgo biloba L.Nature, 1964
- A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue CulturesPhysiologia Plantarum, 1962
- Arginine-requiring Strains of Tissue Obtained from Ginkgo PollenPlant Physiology, 1960
- Production of Large Amounts of Plant Tissue by Submerged CultureScience, 1959