Synthesis of adenosine 5′[(R)α-17O]triphosphate

Abstract

Adenosine 5′[(S)α-thio]triphosphate is converted predominatly into adenosine 5′[γ-¹⁸O]triphosphate by bromine in [¹⁸O]water, but adenosine 5′[(S)α-thio]diphosphate gives adenosine 5′[(R)α-¹⁷O]diphosphate on treatment with bromine in [¹⁷O]water which can be converted enymically into adenosine 5′[(R)α-¹⁷O]-triphosphate.