In vitro radiosensitivity of human fresh T-lymphocytes by colony formation assay using PHA and recombinant interleukin-2.
Open Access
- 1 January 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Radiation Research
- Vol. 28 (3), 221-226
- https://doi.org/10.1269/jrr.28.221
Abstract
In vitro culture conditions for colony formation of human fresh peripheral T-cells using PHA and recombinant Interleukin-2 are defined. Peripheral lymphocytes, from six individuals, were exposed to X or gamma rays in vitro, and dose-survival curves were obtained. The results showed typical sigmoid curves similar to those observed when other mammalian cells are exposed to radiation. The D10 (dose required to kill 90% of the cells) was found to be 3.0 to 3.5 Gy.This publication has 6 references indexed in Scilit:
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