ENZYMATIC SYNTHESIS OF THE SALMONELLA 0-ANTIGEN
Open Access
- 1 November 1964
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 52 (5), 1302-1309
- https://doi.org/10.1073/pnas.52.5.1302
Abstract
Enzymatic synthesis of the galactosyl-mannosyl-rhamnosyl repeating unit of the Salmonella anatum O-antigen from nucleotide sugar precursors is described. Long chains are formed with little or no terminal labeling of the lipopolysaccharide. The product is largely precipitable by anti-O-antiserum and gives rise to the typical oligosaccharide pattern of the antigen following partial acid hydrolysis.Keywords
This publication has 5 references indexed in Scilit:
- Sugar nucleotide transferases in Escherichia coli lipopolysaccharide biosynthesisBiochemical and Biophysical Research Communications, 1964
- Enzymatic incorporation of N-acetylglucosamine into cell wall lipopolysaccharide in a mutant strain of Salmonella typhimuriumBiochemical and Biophysical Research Communications, 1964
- Analysis of the Serologic Determinant Groups of the Salmonella E-Group O-Antigens*Biochemistry, 1963
- Studies on the Chemical Basis of the Phage Conversion of O-Antigens in the E-Group Salmonellae*Biochemistry, 1962
- A simple procedure for the preparation of uridine diphosphogalactoseAnalytical Biochemistry, 1961