Early change in skeletal muscle protein synthesis after limb immobilization of rats
- 1 November 1979
- journal article
- research article
- Published by American Physiological Society in Journal of Applied Physiology
- Vol. 47 (5), 974-977
- https://doi.org/10.1152/jappl.1979.47.5.974
Abstract
The atrophy of skeletal muscle accruing from disuse, or limb immobilization, is caused by a decreased rate of protein synthesis and an increased rate of protein degradation. Currently, little information is available regarding the initial time of the decline in the rate of protein synthesis in skeletal muscle. The purpose of the present study was to determine, as precisely as possible, the time at which the protein synthesis rate first begins to decline in skeletal muscle, utilizing immobilized limbs of rats for a model. A constant-infusion technique employing [14C]tyrosine was used to estimate protein synthesis rates. During the first 6 h of immobilization, a significant decline of 37% in the fractional rate of protein synthesis from the control level of 5.7%/day was observed. These results suggest that very early changes are occurring in molecular events that regulate protein synthesis in disused or immobilized skeletal muscle.This publication has 10 references indexed in Scilit:
- An analysis of errors in estimation of the rate of protein synthesis by constant infusion of a labelled amino acidBiochemical Journal, 1978
- Measurement of the rate of protein synthesis and compartmentation of heart phenylalanineJournal of Biological Chemistry, 1978
- Regulation of protein synthesis in rabbit reticulocyte lysates by the heme-regulated protein kinase: Inhibition of interaction of Met-tRNA f Met binding factor with another initiation factor in formation of Met-tRNA f Met ·40S ribosomal subunit complexesProceedings of the National Academy of Sciences, 1978
- Time course of muscular atrophy during immobilization of hindlimbs in ratsJournal of Applied Physiology, 1977
- MEASUREMENTS OF HALF-LIFE OF RAT CARDIAC MYOSIN HEAVY-CHAIN WITH LEUCYL-TRNA USED AS PRECURSOR POOL1977
- The influence of immobilization and stretch on protein turnover of rat skeletal muscle.The Journal of Physiology, 1977
- Starvation results in decreased initiation factor activity in rat skeletal muscleBiochemical and Biophysical Research Communications, 1976
- ASSESSMENT OF PROTEIN TURNOVER IN PERFUSED RAT-LIVER - EVIDENCE FOR AMINO-ACID COMPARTMENTATION FROM DIFFERENTIAL LABELING OF FREE AND TRANSFER-RNA-BOUND VALINE1976
- Origins and metabolism of the intracellular amino acid pools in rat liver and muscleBiochimica et Biophysica Acta (BBA) - General Subjects, 1967
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951