Thein vivo incorporation of [32P]‐labeled orthophosphate into pyrophosphatidic acid and other phospholipids ofCryptococcus neoformans through cell growth
- 1 October 1977
- Vol. 12 (10), 809-813
- https://doi.org/10.1007/bf02533269
Abstract
Cryptococcus neoformans was cultured in a liquid medium containing [32P]-orthophosphate and harvested at various stages of cell growth. An aliquot of the [32P]-labeled cells was transferred to a nonradioactive medium, and the culture was continued again for some hours. The [32P]-radioactivity composition and the phosphorus composition of individual phospholipids relative to the total phospholipid through the incubation periods were estimated. Although levels of major phospholipids remained constant throughout the cell growth, the distribution pattern of the [32P]-radioactivity of individual phospholipids changed remarkably along with the progress of cell growth. The changing patterns of the specific radioactivities of individual phospholipids through the growth phase demonstrated that phosphatidic acid was one of the most active metabolites in phospholipids and that pyrophosphatidic acid was also metabolically active.This publication has 10 references indexed in Scilit:
- Lipid composition of 30 species of yeastLipids, 1976
- The Distribution of Pyrophosphatidic Acid in NatureThe Journal of Biochemistry, 1975
- Positional Specificity of Fatty Acids in Pyrophosphatidic Acid from Cryptococcus neoformansThe Journal of Biochemistry, 1975
- Changes of lipid composition with growth phase of cryptococcus neoformans.Agricultural and Biological Chemistry, 1975
- Biogenesis of mitochondria. Phospholipid synthesis in vitro by yeast mitochondrial and microsomal fractionsBiochemical Journal, 1974
- Pyrophosphatidic AcidThe Journal of Biochemistry, 1974
- Inositol Phosphorylceramide, a Novel Substance and the Chief Member of a Major Group of Yeast Sphingolipids Containing a Single Inositol PhosphateJournal of Biological Chemistry, 1974
- Application of silica gel‐sintered plate to thin layer lipid chromatographic analysisLipids, 1973
- Phosphorus Assay in Column ChromatographyJournal of Biological Chemistry, 1959