CALCIUM PYROPHOSPHATE DIHYDRATE (CPPD) CRYSTAL-INDUCED CHEMOTACTIC FACTOR - SUBCELLULAR-LOCALIZATION, ROLE OF PROTEIN-SYNTHESIS AND PHAGOCYTOSIS

Abstract

A factor with chemotactic properties for polymorphonuclear leukocytes [human] appears in their lysosomal fraction following phagocytosis of CCPD crystals. The factor, whose appearance was blocked by inhibition of protein synthesis, was estimated to have a MW of 8400 daltons. Inhibition of crystal phagocytosis by cytochalasin B also inhibited the generation of chemotactic factor activity, indicating that ingestion of the crystals by the cell is essential for the generation of the CPPD crystal-induced chemotactic factor. This latter finding provides a clue in the understanding of the development and termination of the acute pseudogout attack.