Relation of R Factor and Chromosomal β-Lactamase with the Periplasmic Space
- 1 March 1974
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 117 (3), 931-939
- https://doi.org/10.1128/jb.117.3.931-939.1974
Abstract
The release of several R factor and chromosomal β-lactamases by osmotic shock treatment was studied. It was found that those β-lactamases with a molecular weight of about 20,000 were released, but those with a molecular weight of about 30,000 to 44,000 were not released during osmotic shock. This differential release did not depend on whether the structural genes were on the chromosome or on the genome of an R factor. The release or retention of the β-lactamases appeared to be a characteristic of the enzyme rather than the host cell since the same results were obtained when the R factors were harbored by a variety of host bacteria. Studies with bacteria which produced more than one β-lactamase showed that each enzyme reacted independently to the presence of other β-lactamases produced by the host bacterium.Keywords
This publication has 24 references indexed in Scilit:
- Characterization of the β-lactamase specified by the resistance factor R-1818 in Escherichia coli K12 and other Gram-negative bacteriaBiochemical Journal, 1971
- Some relationships between R-factor and chromosomal β-lactamase in Gram-negative bacteriaBiochemical Journal, 1971
- The purification and properties of the β-lactamase specified by the resistance factor R-1818 in Escherichia coli and Proteus mirabilisBiochemical Journal, 1971
- Resistance ofEscherichia colito penicillins: IV. Genetic study of mutants resistant to D, L-ampicillin concentrations of 100 μg/ml.Genetics Research, 1968
- The Role of Tris in EDTA Toxicity and Lysozyme LysisJournal of General Microbiology, 1967
- Penicillinase Synthesis Controlled By Infectious R Factors In EnterobacteriaceaeNature, 1965
- Effect of EDTA upon bacterial permeability to benzylpenicillinBiochemical and Biophysical Research Communications, 1965
- TRANSFER OF EPISOMIC ELEMENTS TO PROTEUS II. Nature of lac + Proteus Strains Isolated From Clinical Specimens Journal of Bacteriology, 1964
- Penicillinase from Klebsiella aerogenes. A comparison with penicillinases from gram-positive speciesBiochemical Journal, 1963
- Some Observations on Linkage Effects in Genetic Recombination in Escherichia coli K-12Journal of General Microbiology, 1954