The yeast GAL1-10 upstream activation sequence region readily accepts nucleosomes in vitro
- 1 September 1989
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 28 (18), 7486-7490
- https://doi.org/10.1021/bi00444a048
Abstract
To test if the absence of nucleosomes on the UAS region of the yeast GAL1-10 genes in vivo could be due to a low inherent affinity of this DNA for histones, DNA fragments containing the UAS and various amounts of flanking DNA were reconstituted into chromatin. Restriction enzyme and DNase I digestion analyses show that DNA in the UAS becomes protected against digestion in the reconstitutes. Thus, nucleosomes can assemble on the UAS region in vitro. The level of protection of the UAS and of the flanking DNA regions is comparable and remains so at various levels of nucleosome loading, suggesting that the UAS DNA has no tendency to exclude nucleosomes. In fact, DNase I results suggest that the UAS elements themselves preferentially bind histones.Keywords
This publication has 13 references indexed in Scilit:
- The regulatory protein GAL80 is a determinant of the chromatin structure of the yeast GAL1-10 control region.Journal of Biological Chemistry, 1987
- Distinguishing between mechanisms of eukaryotic transcriptional activation with bacteriophage T7 RNA polymeraseCell, 1987
- DNA sequence-directed nucleosome reconstitution on 5S RNA genes of Xenopus laevis.Molecular and Cellular Biology, 1987
- Competition for formation of nucleosomes on fragmented SV40 DNA: a hyperstable nucleosome forms on the termination regionBiochemistry, 1987
- Reconstitution experiments show that sequence-specific histone-DNA interactions are the basis for nucleosome phasing on mouse satellite DNACell, 1985
- Specific DNA binding of GAL4, a positive regulatory protein of yeastCell, 1985
- A 145-base pair DNA sequence that positions itself precisely and asymmetrically on the nucleosome core.The EMBO Journal, 1984
- Structural features of a phased nucleosome core particle.Proceedings of the National Academy of Sciences, 1983
- A GAL10-CYC1 hybrid yeast promoter identifies the GAL4 regulatory region as an upstream site.Proceedings of the National Academy of Sciences, 1982
- DNA sequence directs placement of histone cores on restriction fragments during nucleosome formationBiochemistry, 1979