DETECTION OF DISSEMINATED TUMOR CELLS IN PERIPHERAL BLOOD
- 1 January 2005
- journal article
- review article
- Published by Taylor & Francis in Critical Reviews in Clinical Laboratory Sciences
- Vol. 42 (2), 155-196
- https://doi.org/10.1080/10408360590913696
Abstract
Metastases are the major cause of cancer-related deaths in patients with solid epithelial malignancies, such as breast, colorectal, and prostate carcinomas. Hematogenous spreading of tumor cells from a primary tumor can be considered as a crucial step in the metastasis cascade leading eventually to the formation of clinically manifest metastases. Consequently, as shown in recent studies, the detection of disseminated tumor cells in peripheral blood might be of clinical relevance with respect to individual patient prognosis and staging or monitoring of therapy. However, the rarity of disseminated tumor cells in peripheral blood renders the application of sensitive techniques mandatory for their detection. The emergence of highly sophisticated reverse transcriptase–polymerase chain reaction (RT-PCR) assays, combining a preanalytical enrichment step with the assessment of multiple molecular tumor markers expressed in disseminated tumor cells, provides a powerful tool in detecting disseminated tumor cells with high sensitivity and specificity. This review will discuss currently used tumor markers as well as experimental means to enhance the sensitivity and specificity of RT-PCR assays to detect disseminated tumor cells in the peripheral blood of patients with breast, colorectal, and prostate cancers, and their clinical relevance assessed in recent studies.Keywords
This publication has 152 references indexed in Scilit:
- Gene expression profiling of colon cancer by DNA microarrays and correlation with histoclinical parametersOncogene, 2004
- Detection of micrometastatic breast cancer by means of real time quantitative RT‐PCR and immunostaining in perioperative blood samples and sentinel nodesInternational Journal of Cancer, 2003
- Surgery‐related shedding of breast cancer cells as determined by RT‐PCR assayJournal of Surgical Oncology, 2003
- Immunobead RT-PCR versus regular RT-PCR amplification of CEA mRNA in peripheral bloodZeitschrift für Krebsforschung und Klinische Onkologie, 2001
- Disseminated tumour cellsVirchows Archiv, 2001
- Clinical Significance of K-Ras Mutations in Intraoperative Tumor Drainage Blood From Patients With Colorectal CarcinomaAnnals of Surgical Oncology, 2001
- REVIEW: Detection of circulating breast cancer cells by reverse transcriptase polymerase chain reaction (RT-PCR)European Journal of Surgical Oncology, 2000
- The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissuesSeminars in Cancer Biology, 1999
- Detection of β-human chorionic gonadotropin mRNA as a marker for cutaneous malignant melanomaInternational Journal of Cancer, 1996
- Carcinoembryonic antigen, a human tumor marker, functions as an intercellular adhesion moleculeCell, 1989