V79 Chinese-hamster cells rendered resistant to high cadmium concentration also become resistant to oxidative stress
- 1 December 1988
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 256 (2), 475-479
- https://doi.org/10.1042/bj2560475
Abstract
Chinese hamster cells (V79) resistant to high concentrations of Cd2+ in the medium were obtained by using the procedure of Beach & Palmiter [(1981) Proc. Natl. Acad. Sci. U.S.A. 78, 2110-2114], which in mouse led to amplification of metallothionein (MT) genes and to an enrichment in cellular MT. The Cd-resistant V79 clones isolated were significantly more resistant than parental cells to oxidative stress by extracellular H2O2 or a mixture of H2O2 and superoxide anion (O2-) generated by xanthine oxidase plus acetaldehyde. On a per-cell basis, there was no difference between the two cells in their total H2O2-decomposing or O2-(-)dismutating activity. The most likely explanation is that an enrichment in MT content in the Cd-resistant cells was responsible for this effect, because of the antioxidant properties already described for this protein.This publication has 19 references indexed in Scilit:
- Iron-mediated induction of sister-chromatid exchanges by hydrogen peroxide and superoxide anionMutation Research, 1987
- Iron(II)-substituted metallothionein: evidence for the existence of iron-thiolate clustersBiochemistry, 1986
- Inhibition of cell membrane lipid peroxidation by cadmium- and zinc-metallothioneinsBiochimica et Biophysica Acta (BBA) - General Subjects, 1986
- METALLOTHIONEINAnnual Review of Biochemistry, 1986
- Evidence that glutathione participates in the induction of a stress proteinBiochemical and Biophysical Research Communications, 1986
- Quantitation of chick tissue zinc-metallothionein by gel electrophoresis and silver stain enhancementComparative Biochemistry and Physiology Part C: Comparative Pharmacology, 1986
- Protection of mammalian cells by o-phenanthroline from lethal and DNA-damaging effects produced by active oxygen speciesBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1985
- Metallothioneins: Proteins in search of functionCell, 1985
- Possible role for metallothionein in protection against radiation-induced oxidative stress. Kinetics and mechanism of its reaction with superoxide and hydroxyl radicalsBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1985
- Transition series metals and sulfhydryl reagents induce the synthesis of four proteins in eukaryotic cellsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1980