An alternatively spliced form of the transcription factor Sp1 containing only a single glutamine-rich transactivation domain.
- 26 September 1995
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 92 (20), 9107-9111
- https://doi.org/10.1073/pnas.92.20.9107
Abstract
Protein-protein interactions involving specific transactivation domains play a central role in gene transcription and its regulation. The promoter-specific transcription factor Sp1 contains two glutamine-rich transcriptional activation domains (A and B) that mediate direct interactions with the transcription factor TFIID complex associated with RNA polymerase II and synergistic effects involving multiple Sp1 molecules. In the present study, we report the complementary DNA sequence for an alternatively spliced form of mouse Sp1 (mSp1-S) that lacks one of the two glutamine-rich activation regions present in the full-length protein. Corresponding transcripts were identified in mouse tissues and cell lines, and an Sp1-related protein identical in size to that predicted for mSp1-S was detected in mouse nuclear extracts. Cotransfection analysis revealed that mSp1-S lacks appreciable activity at promoters containing a single Sp1 response element but is active when multiple Sp1 sites are present, suggesting synergistic interactions between multiple mSp1-S molecules. The absence of a single glutamine-rich domain does not fully explain the properties of the smaller protein and indicates that additional structural features account for its unique transcriptional activity. The functional implications of this alternatively spliced form of Sp1 are discussed.Keywords
This publication has 29 references indexed in Scilit:
- Dual role of TFIIH in DNA excision repair and in transcription by RNA polymerase IINature, 1994
- Inducibility and negative autoregulation of CREM: An alternative promoter directs the expression of ICER, an early response repressorCell, 1993
- Molecular cloning and functional analysis of Drosophila TAF110 reveal properties expected of coactivatorsCell, 1993
- Sequence discrimination by alternatively spliced isoforms of a DNA binding zinc finger domainScience, 1992
- An Origin of DNA Replication and a Transcription Silencer Require a Common ElementScience, 1992
- Localization of the gene for the trans-acting transcription factor Sp1 to the distal end of mouse chromosome 15Genomics, 1990
- GC box binding induces phosphorylation of Sp1 by a DNA-dependent protein kinaseCell, 1990
- Dissection of functional domains of the pituitary-specific transcription factor GHF-1Nature, 1989
- Synergistic activation by the glutamine-rich domains of human transcription factor Sp1Cell, 1989
- A simple phase-extraction assay for chloramphenicol acyltransferase activityGene, 1988