Distribution of polyamines in methanogenic bacteria
- 1 June 1983
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 154 (3), 1315-1322
- https://doi.org/10.1128/jb.154.3.1315-1322.1983
Abstract
Members of all 4 families of methanogenic bacteria were analyzed for polyamine concentrations. High-performance liquid chromatography analysis of dansylated cell extracts revealed typical polyamine patterns for each family. Methanobacteriaceae (family I) were characterized by very low polyamine concentrations; Methanococcaceae (family II) were characterized by putrescine and high spermidine concentrations; Methanomicrobiaceae (family III) were characterized by the presence of putrescine, spermidine and sym-homospermidine; Methanosarcinaceae (family IV) contained only high concentrations of sym-homospermidine in addition to putrescine. The highest polyamine concentration was found in Methanosarcina barkeri Julich, with 0.35% putrescine in the dry cell material. The polyamine distribution found coincides with the dendrogram based on comparative cataloguing of 16S rRNA and offers a new, rapid chemotaxonomic method for characterizing methanogenic bacteria. Variation of the growth substrates (H2-CO2, methanol, acetate and trimethylamine) for M. barkeri resulted in quantitative but not qualitative differences in polyamine composition.This publication has 37 references indexed in Scilit:
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