The oxidation of glycerol by mycobacteria
- 1 September 1953
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 55 (2), 320-328
- https://doi.org/10.1042/bj0550320
Abstract
Glycerol is oxidized and assimilated by washed suspensions of Mycobacterium smegmatis and M. butyricum. Oxidation is inhibited completely by cyanide and partially by selective agents such as arsenite, iodoacetate and fluoride. Pyruvate accumulates extracellularly in the presence of arsenite only. Study of dialyzed extracts shows that the primary step in the degradation of glycerol is phosphorylation with adenosine triphosphate. Glycerophosphate is oxidized subsequently to triose phosphate and broken down to pyruvate by reactions of the Embden-Meyerhof scheme.Keywords
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