In Vitro Studies of Chemical Mutagens and Carcinogens. I. Stability Studies in Cell Culture Medium2
- 1 September 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in JNCI Journal of the National Cancer Institute
- Vol. 59 (3), 941-944
- https://doi.org/10.1093/jnci/59.3.941
Abstract
A quantitative microbial mutagenesis assay was used to study the stability of known mutagenic and carcinogenic compounds in cell culture medium. Ten direct-acting carcinogens, when incubated in culture medium with 15% fetal bovine serum at pH 7-2-7-4 and 37° C, became inactive at varying rates. Biologic half-lives of the test compounds ranged from 8 minutes to 67 hours. In contrast, six procarcinogens showed no significant inactivation after 3 weeks' incubation. The biologic half-lives of each compound were presented, and the significance of these findings as they relate to cell culture carcinogenesis and mutagenesis assays was discussed.Keywords
This publication has 2 references indexed in Scilit:
- MUTAGENICITY AND CYTOTOXICITY OF BENZO(A)PYRENE BENZO-RING EPOXIDES1976
- In Vitro Transformation of Normal Cells to Tumor Cells by Carcinogenic Hydrocarbons2JNCI Journal of the National Cancer Institute, 1965