A BIOLOGICAL ASSAY FOR CALCITONIN
- 1 November 1965
- journal article
- research article
- Published by Bioscientifica in Journal of Endocrinology
- Vol. 33 (3), 469-475
- https://doi.org/10.1677/joe.0.0330469
Abstract
SUMMARY: (1) Calcitonin preparations from acetone-dried thyroid were administered to rats by various routes. (2) Intravenous administration, especially by infusion, produced a much greater fall in plasma calcium than s.c. or i.p. injection. (3) The log dose-effect curves after i.v. injection or infusion showed no evidence of non-linearity over a 100-fold dose range and had highly significant slopes. (4) The potency ratio of two preparations was estimated by means of a (2+2) assay design using both i.v. infusion and single i.v. injection. There was satisfactory agreement. (5) The i.v. injection method is recommended for the routine assay of calcitonin. A simple assay schedule is given in the Appendix. (6) A unit of calcitonin activity is defined in terms of a standard preparation.This publication has 7 references indexed in Scilit:
- Thyrocalcitonin: Hypocalcemic Hypophosphatemic Principle of the Thyroid GlandScience, 1964
- Further Studies on the Isolation and Characterization of Parathyroid PolypeptidesJournal of Biological Chemistry, 1964
- Calcitonin Production and the Mitochondrion-Rich Cells of the Dog ThyroidNature, 1964
- Extraction and Purification of CalcitoninNature, 1964
- Thyroid origin of CalcitoninNature, 1964
- Thyroid Hypocalcemic Principle and Recurrent Laryngeal Nerve Injury as Factors Affecting the Response to Parathyroidectomy in Rats1Endocrinology, 1963
- Evidence for Calcitonin—A New Hormone from the Parathyroid That Lowers Blood CalciumEndocrinology, 1962