The inhibition of glutamate dehydrogenase by l-serine O-sulphate and related compounds and by photo-oxidation in the presence of Rose Bengal
- 1 July 1971
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 123 (3), 421-426
- https://doi.org/10.1042/bj1230421
Abstract
1. Glutamate dehydrogenase was inhibited by l-serine O-sulphate, β-chloro-l-alanine, O-phospho-l-serine and β-chloro-l-alanine methyl ester. With the exception of β-chloro-l-alanine methyl ester which was an irreversible inhibitor, it was possible to reverse the inhibitory effects by dialysis. 2. Both NAD+ and glutamate afford some protection against the inhibition due to the methyl ester. No change in the normal stimulatory effect exhibited by ADP was observed in the presence of β-chloro-l-alanine methyl ester but the effect due to GTP was modified. 3. Irradiation of glutamate dehydrogenase in the presence of Rose Bengal produced rapid inactivation. Amino acid analysis of the inactivated enzyme showed that eight histidine residues had been destroyed in the process.Keywords
This publication has 11 references indexed in Scilit:
- The Reaction of Glutamate Dehydrogenase with 4‐Iodoacetamido Salicylic AcidEuropean Journal of Biochemistry, 1970
- The mechanism of glutamate dehydrogenase reaction. 3. The binding of ligands at multiple subsites and resulting kinetic effects.1970
- Effects of Bromopyruvate on the Control and Catalytic Properties of Glutamate DehydrogenaseEuropean Journal of Biochemistry, 1969
- Reaction of L-serine O-sulfate with aspartate aminotransferaseBiochemistry, 1969
- A rat liver system that catalyses a pyridoxal phosphate-independent αβ-eliminationBiochemical Journal, 1969
- Chemical modification of glutamate dehydrogenase by 2,4,6-trinitrobenzenesulphonic acidBiochemical Journal, 1969
- A peptide containing a reactive lysyl group from ox liver glutamate dehydrogenaseBiochemical Journal, 1969
- The involvement of a tryptophan residue of glutamate dehydrogenase in the binding of L-glutamateBiochemical and Biophysical Research Communications, 1965
- The metabolism of potassium l-serine O[35S]-sulphate in the ratBiochemical Journal, 1962
- The spectrophotometric determination of tyrosine and tryptophan in proteinsBiochemical Journal, 1946