Summary In a 15-month study, the effects of prepubertal gonadectomy on skeletal growth, weight gain, food intake, body fat, secondary sex characteristics, and behavioral development were investigated in 32 mixed-breed dogs. Male and female pups from 5 litters were randomly allotted to 3 groups: group 1, neuter at 7 weeks (n = 14); group II, neuter at 7 months (n = 8); and group III, sexually intact dogs (n = 10). Growth plate closure was delayed (group 1 vs group III; P < 0.000001; group II vs group III, P < 0.000001) in all neutered dogs, as compared with sexually intact dogs. Growth plate closure was delayed longer (group I vs group II, P < 0.000045) in dogs neutered at 7 weeks old, compared with dogs neutered at 7 months old. The rate of growth was unaffected by gonadectomy, but the extended growth period resulted in greater final radial/ulnar length in all male dogs and bitches neutered at 7 weeks. Gonadectomy did not influence food intake, weight gain, or back-fat depth. Penile development was immature in the adult group- I males (Mean ± sem diameter of pars glandis = 11.1 ± 1.0 mm), compared with adult group-II (16.3 ± 0.5 mm) and group-III (21.0 ± 2.2 mm) males. Subjectively, the prepuce and os penis of the group-I males were immature, compared with those of group-II and group-III males. Vulvar development in group-I and group-II bitches was less mature than vulvar development in the sexually intact bitches. Of 7 behavioral characteristics assessed, only general activity and excitability rated differently among treatment groups. All neutered dogs were judged to be more active (group I, P < 0.004) than sexually intact dogs. Group-I males were judged to be more excitable (P < 0.02) than group-III males. It was concluded that with respect to skeletal, physical, and behavioral development, the effect of neutering pups at 7 weeks old was similar to that of neutering pups at 7 months old.