Asymmetry in active complexes of FLP recombinase.
- 15 August 1995
- journal article
- research article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 9 (16), 2053-2064
- https://doi.org/10.1101/gad.9.16.2053
Abstract
The FLP recombinase promotes a site-specific recombination reaction in the 2mu plasmid of yeast. The protein-DNA complex that carries out the reaction is asymmetric. Three FLP monomers bound to specific FLP-recognition sequences are required to efficiently carry out one set of reciprocal DNA cleavage and strand exchange events on a Holliday junction substrate. If a fourth monomer plays an auxiliary role in the reaction, it is bound without sequence specificity. The data suggest a modified model for cleavage of DNA in trans by the FLP recombinase that might help reconcile some seemingly conflicting resulted obtained with integrase class recombinases.Keywords
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