Superficial treatment of mammalian cells using plasma needle

Abstract

Interactions of a small-size, non-thermal plasma (plasma needle) with living cells in culture are studied. We have demonstrated the non-destructive character of the plasma needle: under moderate conditions (low-power and low concentration of molecular species) the plasma needle does not heat biological samples and does not induce cell death. Treatment of living cells is restricted to the cell exterior (membrane). As a result of the interactions of plasma radicals with cell adhesion molecules, cell attachment is temporarily interrupted; the loose cells can be removed, reattached or transferred. This effect may prove very useful in fine surgery, where a part of the tissue must be removed with high-precision, without damage to the adjacent cells and without inflammatory reaction.