Concentration of 17β-Estradiol Using an Immunoaffinity Porous Hollow-Fiber Membrane
- 29 August 2002
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 74 (19), 4933-4936
- https://doi.org/10.1021/ac020141e
Abstract
We describe attempts to achieve high throughput of 17β-estradiol (E2) analysis, including the development of an immunocleanup membrane using polyclonal antibodies and an enzyme-linked immunosorbent assay (ELISA) using monoclonal antibodies. An epoxy-group-containing monomer, glycidyl methacrylate (GMA), was graft-polymerized onto a porous hollow-fiber membrane. Subsequently, anti-estrogen (ES) antibody, as a ligand, was coupled with the epoxy group. The ligand density ranged from 3.1 to 5.8 mg/g of the GMA-grafted porous hollow-fiber membrane. A 1.0 μg/L E2 solution was forced to permeate through pores rimmed by the anti-ES-antibody-immobilized polymer chains, at a constant permeation rate. A breakthrough curve, that is, the change in the E2 concentration of the effluent penetrating the outside of the hollow fiber with a change of the effluent volume, was determined. Bound E2 in amounts ranging from 0.42 to 0.80 μg was quantitatively eluted with 3−5 mL of methanol in the permeation mode. The higher permeation rate of the E2 solution resulted in the higher overall binding rate of E2 to the anti-ES-antibody-immobilized porous hollow-fiber membrane because of the negligible diffusional mass-transfer resistance of E2 to the antibody.Keywords
This publication has 16 references indexed in Scilit:
- Analysis and environmental levels of endocrine-disrupting compounds in freshwater sedimentsTrAC Trends in Analytical Chemistry, 2001
- Immunohistochemical localization of cytochrome P4501A induced by 3,3′,4,4′,5‐pentachlorobiphenyl (PCB 126) in multiple organs of northern leopard frogs, Rana pipiensEnvironmental Toxicology and Chemistry, 2001
- Developmental Dysplasia of the Hip Practice Guideline: Technical ReportPediatrics, 2000
- An Enzyme-Linked Immunosorbent Assay for Detection of Linear Alkylbenzene Sulfonate: Development and Field StudiesEnvironmental Science & Technology, 1998
- Alterations in steroidogenesis in alligators (Alligator mississippiensis) exposed naturally and experimentally to environmental contaminants.Environmental Health Perspectives, 1997
- Comparison of protein adsorption by anion-exchange interaction onto porous hollow-fiber membrane and gel bead-packed bedJournal of Membrane Science, 1996
- Highly Efficient Enzyme Recovery Using a Porous Membrane with Immobilized Tentacle Polymer ChainsBio/Technology, 1995
- Binding of Lysozyme onto a Cation‐Exchange Microporous Membrane Containing Tentacle‐Type Grafted Polymer BranchesBiotechnology Progress, 1994
- Triphase catalysis for recovery of phenol from an aqueous alkaline streamIndustrial & Engineering Chemistry Research, 1992
- Introduction of a high-density chelating group into a porous membrane without lowering the fluxIndustrial & Engineering Chemistry Research, 1991