An Improved Technique for Perfusion of the Guinea Pig Placenta in situ Giving Viable Conditions Demonstrated by Placental Transport of Amino Acids (L- and D-Alanine)

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Abstract
An improved technique for perfusing the fetal guinea pig placenta in situ is described. The result shows linear pressure-flow relations in the range of 15–50 mm Hg, assumed to be physiological. The preparation shows impermeability for trypan blue. The placental transfer is stereoselective for L-alanine compared with D-alanine. This transfer is ouabain-sensitive, and proceeds against a concentration gradient.