Purification of chicken liver ribonucleases by affinity chromatography with UMP-Sepharose (nucleosides and nucleotides. LII).
- 1 January 1984
- journal article
- research article
- Published by Pharmaceutical Society of Japan in CHEMICAL & PHARMACEUTICAL BULLETIN
- Vol. 32 (10), 4054-4060
- https://doi.org/10.1248/cpb.32.4054
Abstract
A procedure for the preparation of chicken liver RNases is described, involving affinity chromatography on Sepharose coupled with 5''-amino-5''-deoxyuridine 2''(3'')-phosphate. Two kinds of RNases having acid pH optima were obtained. One RNase exhibited preferential nucleolytic activity toward poly(C) rather than poly(U) and the other was specific for poly(U). The former RNase may be identical to that reported by Levy et al. and the latter resembles acid RNase detected in several mammalian tissues.This publication has 1 reference indexed in Scilit:
- Identification of a cytidine-specific ribonuclease from chicken liver.Journal of Biological Chemistry, 1980