Dihydrofolate reductase: the amino acid sequence of the enzyme from a methotrexate-resistant mutant of Escherichia coli
- 4 April 1978
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 17 (7), 1328-1337
- https://doi.org/10.1021/bi00600a030
Abstract
The determination of the amino acid sequence of the enzyme dihydrofolate reductase (5,6,7,8-tetrahydrofolate:NADP+ oxidoreductase, EC 1.5.1.3) from a mutant of E. coli B is described. The 159 residues were positioned by automatic Edman degradation of the whole protein, of the reduced and alkylated CNBr fragments, and of selected tryptic, chymotryptic and thermolytic digestion products. An N-bromosuccinimide produced fragment of the largest CNBr peptide was also used in the sequence determination.Keywords
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