Free-Living Rhizobium Strain Able To Grow on N 2 as the Sole Nitrogen Source

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Abstract
A Rhizobium strain isolated from stem nodules of the legume Sesbania rostrata was shown to grow on atmospheric nitrogen (N 2 ) as the sole nitrogen source. Non-N 2 -fixing mutants isolated directly on agar plates formed nodules that did not fix N 2 when inoculated into the host plant.