Expression of a proteasome α-type subunit gene during tobacco development and senescence
- 1 January 1999
- journal article
- Published by Springer Nature in Plant Molecular Biology
- Vol. 39 (2), 325-333
- https://doi.org/10.1023/a:1006102110889
Abstract
Proteasomes degrade specific proteins that have been targeted for proteolysis by ubiquitination. In animals and yeast nuclear-localised proteasomes play a role in regulating the cell cycle, and other developmental processes, via control of the levels of regulatory nuclear proteins such as cyclins and transcription factors. A cDNA, NtPSA1, isolated from tobacco styles was found to have high similarity to human and yeast genes, PRCI−human and PRCI−yeast with 63.4% and 51.6% overall identity respectively. These genes are believed to encode non-catalytic α-type subunits of 26S proteasomes and like NtPSA1 have putative nuclear localisation signals. NtPSA1 RNA was found to accumulate to varying levels in different parts of the plant and at different developmental stages. In particular, the level of NtPSA1 RNA was high in young dividing and expanding tissues, and declined during the senescence of both leaves and flowers. These results suggest that a role of proteasomes in plant nuclei may be to regulate developmental events by controlling the levels of regulatory proteins in proliferating and developing tissues, rather than to degrade and recycle proteins during senescence.Keywords
This publication has 53 references indexed in Scilit:
- IKK-1 and IKK-2: Cytokine-Activated IκB Kinases Essential for NF-κB ActivationScience, 1997
- Phosphorylation-dependent degradation of the cyclin-dependent kinase inhibitor p27Kip1The EMBO Journal, 1997
- Complex mechanisms for c-fos and c-jun degradationMolecular Biology Reports, 1997
- Pollination-enhanced expression of a receptor-like protein kinase related gene in tobacco stylesPlant Molecular Biology, 1997
- Prosomes (proteasomes) changes during differentiation are related to the type of inducerMolecular Biology Reports, 1997
- Subpopulations of proteasomes in rat liver nuclei, microsomes and cytosolBiochemical Journal, 1996
- [Degradation mechanism of cell cycle factors by the proteasome].1996
- Phosphorylation of Proteasome Substrate by a Protein-Kinase Associated with the 26S-Proteasome Is Linked to the ATP-Dependent Proteolysis of the 26S-ProteasomeBiochemical and Biophysical Research Communications, 1995
- Tissue-specific expression of the subunits of chick 20S proteasomes.1994
- Purification and initial characterization of the proteasome from the higher plant Spinacia oleracea.Journal of Biological Chemistry, 1992