CELL SURFACE IMMUNOGLOBULIN
Open Access
- 1 June 1974
- journal article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 139 (6), 1599-1620
- https://doi.org/10.1084/jem.139.6.1599
Abstract
A new method for the detection of cell surface immunoglobulin labeled with isotopic precursors is described. The method consists of the aggregation of surface Ig on cells with specific antibody (heterologous) and the subsequent removal of antigen-antibody complexes by the combination of high speed centrifugation and immunoprecipitation of remaining soluble complexes using antibody to the heterologous Ig. Using this method, the kinetics of appearance of cell surface Ig and its turnover were studied in murine splenocytes. The results suggest that cell surface Ig is synthesized and transported in the same manner as secretory Ig rather than being synthesized on the plasma membrane. The turnover of intracellular and cell surface Ig in lymphocytes is slow. In contrast, intracellular Ig in plasma cells is rapidly secreted and usually without a cell surface phase. Cell surface Ig was shown to be radiolabeled with [(3)H]glucosamine, -galactose, and -fucose. The proportion of cell surface to intracellular (nonsurface) Ig labeled with these precursors suggests the same sequence of addition of sugars to Ig destined to be on the surface of lymphocytes as with Ig which will be secreted by plasma cells. Results with this new method also confirm earlier conclusions based on experiments using cell surface iodination: 8S IgM is the predominant Ig on the surface of murine splenocytes and the molecule appears to be attached by its micro-chains.Keywords
This publication has 42 references indexed in Scilit:
- CELL SURFACE IMMUNOGLOBULINThe Journal of Experimental Medicine, 1974
- Synthesis, Surface Deposition and Secretion of Immunoglobulin M in Bone Marrow-Derived Lymphocytes Before and After Mitogenic StimulationImmunological Reviews, 1973
- Synthesis, Transport, Dynamics and Fate of Cell Surface Ig and Alloantigens in Murine LymphocytesImmunological Reviews, 1973
- LIGAND-INDUCED MOVEMENT OF LYMPHOCYTE MEMBRANE MACROMOLECULESThe Journal of Experimental Medicine, 1972
- CELL SURFACE IMMUNOGLOBULINThe Journal of Experimental Medicine, 1972
- VARIATION IN ACCESSIBLE CELL SURFACE IMMUNOGLOBULIN AMONG ANTIBODY-FORMING CELLSThe Journal of Experimental Medicine, 1972
- IMMUNOGLOBULIN SYNTHESIS AND SECRETIONThe Journal of Experimental Medicine, 1971
- Intracellular transport and secretion of an immunoglobulin light chainBiochemistry, 1971
- Intracellular events underlying synthesis and secretion of immunoglobulinCellular Immunology, 1970
- Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gelsBiochemical and Biophysical Research Communications, 1967