MARKER EFFECTS ON REVERSION OF T4rII MUTANTS
Open Access
- 29 November 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in Genetics
- Vol. 84 (3), 423-436
- https://doi.org/10.1093/genetics/84.3.423
Abstract
The frequencies of 2-aminopurine- and 5-bromouracil-induced A:T → G:C transitions were compared at nonsense sites throughout the rII region of bacteriophage T4. These frequencies are influenced both by adjacent base pairs within the nonsense codons and by extracodonic factors. Following 2AP treatment, they are high in amber (UAG) and lower in opal (UGA) codons than in allelic ochre (UAA) codons. In general, 5BU-induced transitions are more frequent in both amber and opal codons than in the allelic ochre codons. 2AP- and 5BU-induced transition frequencies in the first and third positions of opal codons are correlated with those in the corresponding positions of the allelic ochre codons. Similarly, the frequencies of 2AP-induced transition in the first and second positions of amber codons and their ochre alleles are correlated. However, there is little correlation between the frequencies of 5BU-induced transitions in the first and second positions of allelic amber and ochre codons.This publication has 4 references indexed in Scilit:
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- Mechanism of Ribonucleic Acid Polymerase Action. Effect of Nearest Neighbors on Competition between Uridine Triphosphate and Uridine Triphosphate Analogs for Incorporation into Ribonucleic AcidBiochemistry, 1967
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- The specific mutagenic effect of base analogues on Phage T4Journal of Molecular Biology, 1959