DNA counterion current and saturation examined by a MEMS-based solid state nanopore sensor
- 23 June 2006
- journal article
- Published by Springer Nature in Biomedical Microdevices
- Vol. 8 (3), 263-269
- https://doi.org/10.1007/s10544-006-9144-x
Abstract
Reports of DNA translocation measurements have been increasing rapidly in recent years due to advancements in pore fabrication and these measurements continue to provide insight into the physics of DNA translocations through MEMS based solid state nanopores. Specifically, it has recently been demonstrated that in addition to typically observed current blockages, enhancements in current can also be measured under certain conditions. Here, we further demonstrate the power of these nanopores for examining single DNA molecules by measuring these ionic currents as a function of the applied electric field and show that the direction of the resulting current pulse can provide fundamental insight into the physics of condensed counterions and the dipole saturation in single DNA molecules. Expanding on earlier work by Manning and others, we propose a model of DNA counterion ionic current and saturation of this current based on our experimental results. The work can have broad impact in understanding DNA sensing, DNA delivery into cells, DNA conductivity, and molecular electronics.Keywords
This publication has 26 references indexed in Scilit:
- Salt Dependence of Ion Transport and DNA Translocation through Solid-State NanoporesNano Letters, 2005
- Stretching DNA Using the Electric Field in a Synthetic NanoporeNano Letters, 2005
- Detecting Single Stranded DNA with a Solid State NanoporeNano Letters, 2005
- Probing Single DNA Molecule Transport Using Fabricated NanoporesNano Letters, 2004
- Atomic Layer Deposition to Fine-Tune the Surface Properties and Diameters of Fabricated NanoporesNano Letters, 2004
- Coarse-grained simulation of polymer translocation through an artificial nanoporePolymer, 2004
- Fabrication of solid-state nanopores with single-nanometre precisionNature Materials, 2003
- Polyelectrolytes in Electric FieldsThe Journal of Physical Chemistry B, 2003
- An atomic force microscope estimation of the point of zero charge of silicon insulatorsSensors and Actuators B: Chemical, 1998
- The molecular theory of polyelectrolyte solutions with applications to the electrostatic properties of polynucleotidesQuarterly Reviews of Biophysics, 1978