Direct 1H NMR evidence for conversion of β‐d‐cellobiose to cellobionolactone by cellobiose dehydrogenase from Phanerochaete chrysosporium
- 29 August 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 351 (1), 128-132
- https://doi.org/10.1016/0014-5793(94)00847-7
Abstract
The α- and β-anomers of d-cellobiose were resolved by 1H NMR spectroscopy. Addition of cellobiose dehydrogenase purified from the white-rot P. chrysosporium led to selective conversion of β-d-cellobiose. The product was identical to cellobionolactone as synthesized from Ca-cellobionate. Overnight incubation of the product led to an altered NMR spectrum, which was also obtained by incubation of cellobionolactone. The new spectrum matched that for Ca-cellobionate. The instability of cellobionolactone explains the detection of cellobionic acid as product in earlier studies.Keywords
This publication has 31 references indexed in Scilit:
- Pathways for production of Fenton's reagent by wood-rotting fungiFEMS Microbiology Reviews, 1994
- Purification and Characterization of Cellobiose Dehydrogenase, a Novel Extracellular Hemoflavoenzyme from the White-Rot Fungus Phanerochaete chrysosporiumArchives of Biochemistry and Biophysics, 1993
- Continuous monitoring of cellulose oxidation by cellobiose oxidase fromPhanerochaete chrysosporiumFEMS Microbiology Letters, 1992
- Evidence that cellobiose:quinone oxidoreductase from Phanerochaete chrysosporium is a breakdown product of cellobiose oxidaseBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1992
- Cellobiose dehydrogenases of Sporotrichum (Chrysosporium) thermophileEuropean Journal of Biochemistry, 1991
- Some properties of cellobiose oxidase from the white-rot fungus Sporotrichum pulverulentumBiochemical Journal, 1985
- Conversion of cellulose to sugars and cellobionic acid by the extracellular enzyme system of Chaetomium cellulolyticumBiotechnology Letters, 1982
- Cellobiose Oxidase, Purification and Partial Characterization of a Hemoprotein from Sporotrichum pulverulentumEuropean Journal of Biochemistry, 1978
- Purification and Properties of Cellobiose:Quinone Oxidoreductase from Sporotrichum pulverulentum.Acta Chemica Scandinavica, 1975
- Oxidation: An important enzyme reaction in fungal degradation of celluloseFEBS Letters, 1974