The C-terminal region of cellular Qin oligomerizes: correlation with oncogenic transformation and transcriptional repression
- 3 April 2003
- journal article
- Published by Springer Nature in Oncogene
- Vol. 22 (13), 1908-1915
- https://doi.org/10.1038/sj.onc.1206307
Abstract
Expression of the oncoprotein Qin induces tumors in chickens and oncogenic transformation of chicken embryo fibroblasts in culture. We performed a detailed deletion analysis of the C-terminal region of Qin (amino acids 246-451, extending from the winged helix domain to the C-terminus) and identified amino acids 246-379 as important for transformation. The same region mediates homo-oligomerization of Qin as documented in vitro by GST pulldowns and in vivo by coimmunoprecipitation. A 60 amino-acid region within the oligomerization domain is necessary and sufficient for transcriptional repression induced by Qin.Keywords
This publication has 30 references indexed in Scilit:
- The Winged-Helix Protein Brain Factor 1 Interacts with Groucho and Hes Proteins To Repress TranscriptionMolecular and Cellular Biology, 2001
- Oncogenic transformation by the FOX protein Qin requires DNA bindingOncogene, 2000
- BF-1 Interferes with Transforming Growth Factor β Signaling by Associating with Smad PartnersMolecular and Cellular Biology, 2000
- Winged helix proteinsCurrent Opinion in Structural Biology, 2000
- Structural basis of DNA recognition by the heterodimeric cell cycle transcription factor E2F-DPGenes & Development, 1999
- Fusion of a fork head domain gene to PAX3 in the solid tumour alveolar rhabdomyosarcomaNature Genetics, 1993
- Co-crystal structure of the HNF-3/fork head DNA-recognition motif resembles histone H5Nature, 1993
- Cytogenetic studies in subgroups of rhabdomyosarcomaGenes, Chromosomes and Cancer, 1992
- Chromosomal Translocation t(1;13)(p36;q14) in a Case of RhabdomyosarcomaGenes, Chromosomes and Cancer, 1991
- Variant Translocations of Chromosome 13 in Alveolar RhabdomyosarcomaGenes, Chromosomes and Cancer, 1991