Enhancing the first enzymatic step in the histidine biosynthesis pathway increases the free histidine pool and nickel tolerance in Arabidopsis thaliana
- 16 November 2004
- journal article
- Published by Wiley in FEBS Letters
- Vol. 578 (1-2), 128-134
- https://doi.org/10.1016/j.febslet.2004.10.086
Abstract
Naturally selected nickel (Ni) tolerance in Alyssum lesbiacum has been proposed to involve constitutively high levels of endogenous free histidine. Transgenic Arabidopsis thaliana expressing a Salmonella typhimurium ATP phosphoribosyl transferase enzyme (StHisG) resistant to feedback inhibition by histidine contained approximately 2-fold higher histidine concentrations than wild type plants. Under exposure to a toxic Ni concentration, biomass production in StHisG expressing lines was between 14- and 40-fold higher than in wild-type plants. This suggested that enhancing the first step in the histidine biosynthesis pathway is sufficient to increase the endogenous free histidine pool and Ni tolerance in A. thaliana.Keywords
This publication has 41 references indexed in Scilit:
- Functional Expression of a Bacterial Heavy Metal Transporter in Arabidopsis Enhances Resistance to and Decreases Uptake of Heavy MetalsPlant Physiology, 2003
- Engineering tolerance and accumulation of lead and cadmium in transgenic plantsNature Biotechnology, 2003
- Increased cadmium tolerance and accumulation by plants expressing bacterial arsenate reductaseNew Phytologist, 2003
- The Role of Free Histidine in Xylem Loading of Nickel inAlyssum lesbiacum and Brassica junceaPlant Physiology, 2003
- An inexpensive alternative to glassmilk for DNA purificationTrends in Genetics, 1995
- Isolation and Characterization of cDNAs Encoding Imidazoleglycerolphosphate Dehydratase from Arabidopsis thalianaPlant Physiology, 1994
- Tobacco mosaic virus infection of transgenic Nicotiana tabacum plants is inhibited by antisense constructs directed at the 5′ region of viral RNAGene, 1993
- Purification and properties of glutamine phosphoribosylpyrophosphate amidotransferase from Bacillus subtilisBiochemistry, 1981
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970