14 C Incorporation into the Fatty Acids and Aliphatic Hydrocarbons of Sarcina lutea
Open Access
- 1 August 1967
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 94 (2), 349-358
- https://doi.org/10.1128/jb.94.2.349-358.1967
Abstract
An initial investigation into the mechanism of hydrocarbon biosynthesis in Sarcina lutea was performed by measuring the amounts of 14C incorporated into the hydrocarbons and fatty acids by use of a combination gas chromatograph and high-temperature gas-flow ionization apparatus. Uniformly labeled l-isoleucine-14C was predominantly incorporated into the anteiso-branched chains. Palmitate-16-14C gave evidence that a direct correlation may exist between the nonpolar end of the palmitate and the biosynthesis of hydrocarbons and carotenoids. The label from palmitate-1-14C was incorporated into the various hydrocarbon groups as a compound, derived from the polar end of the palmitate, consisting of more than two carbon atoms. Palmitate-16-14C and -1-14C gave no detectable evidence that transformed products were incorporated into other fatty acids. Sodium acetate-2-14C and uniformly labeled l-leucine-14C gave evidence of a nonspecific incorporation into both the aliphatic hydrocarbons and fatty acids of Sarcina lutea.This publication has 13 references indexed in Scilit:
- Biosynthesis of branched-chain fatty acids V. Microbial stereospecific syntheses of d-12-methyltetradecanoic and d-14-methylhexadecanoic acidsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1966
- Biosynthesis of Wax in Brassica oleracea. Relation of Fatty Acids to Wax*Biochemistry, 1966
- BIOSYNTHESIS OF BRANCHED-CHAIN FATTY ACIDS: IV. FACTORS AFFECTING RELATIVE ABUNDANCE OF FATTY ACIDS PRODUCED BY BACILLUS SUBTILISCanadian Journal of Microbiology, 1966
- Biosynthesis and Function of Carotenoid Pigments in MicroorganismsAnnual Review of Microbiology, 1965
- Biosynthesis of Wax in Brassica oleracea*Biochemistry, 1965
- LIPIDS OFSARCINA LUTEAIIJournal of Bacteriology, 1964
- Synthesis of wax in the honeybee (Apis mellifera L.)Journal of Insect Physiology, 1964
- LIPIDS OF SARCINA LUTEA IJournal of Bacteriology, 1964
- The role of isoleucine in the biosynthesis of branched-chain fatty acids by micrococcus lysodeikticusBiochemical and Biophysical Research Communications, 1961
- Biogenetische Studien, V. Über die Biogenese von n‐Heptan in Pinus jeffreyi MurrEuropean Journal of Inorganic Chemistry, 1960