Penicillin: analysis of the crude product by partition chromatography. 2. Chromatographic analysis of the penicillins from two strains of Penicillium notatum

Abstract

Partition chromatography with a silica-phosphate buffer-solvent system shows the existence of 5 penicillins. The main antibacterial substance produced by the F4 strain of P. notatum grown on a modified Czapek-Dox medium is penicillin I which is accompanied by small quantities of 3 other penicillins. The main antibacterial substance produced by the N.R.R.L. no. 832 strain of P. notatum grown submerged in a brown sugar-corn steep liquor medium is penicillin II, different from any of the above. There is a differential response of 2 organisms, Staphylococcus aureus and Bacillus subtilis, to the action of F4 penicillin and 832 penicillin.