METHODS OF MEASURING BLOOD MEAL SIZE AND PROTEINASE ACTIVITY FOR DETERMINING EFFECTS OF MATED STATE ON DIGESTIVE PROCESSES OF FEMALE AEDES AEGYPTI (L.) (DIPTERA: CULICIDAE)

Abstract

Two techniques for measuring size of ingested blood meal and 5 methods of measuring proteinase activity in the midgut of Aedes aegypti are compared. Protein, measured after trichloroacetic acid precipitation, was found to be most suitable for determining blood meal size and loss of protein from the gut. Of 5 proteinase substrates tested (BAPNA (benzoyl-DL-arginine-p-nitroanilide), hemoglobin, casein, azocasein, and hide powder azure), BAPNA was considered most suitable for trypsin measurements. These techniques were used to show that virgin females, 6 and 10 days after eclosion, consumed smaller meals than mated females. Virgin females also showed delays in both onset of protein loss from the gut and the increase in proteinase activity after blood feeding.