Fourier transform ion cyclotron resonance mass spectrometry: A primer

Abstract

This review offers an introduction to the principles and generic applications of FT‐ICR mass spectrometry, directed to readers with no prior experience with the technique. We are able to explain the fundamental FT‐ICR phenomena from a simplified theoretical treatment of ion behavior in idealized magnetic and electric fields. The effects of trapping voltage, trap size and shape, and other nonidealities are manifested mainly as perturbations that preserve the idealized ion behavior modified by appropriate numerical correction factors. Topics include: effect of ion mass, charge, magnetic field, and trapping voltage on ion cyclotron frequency; excitation and detection of ICR signals; mass calibration; mass resolving power and mass accuracy; upper mass limit(s); dynamic range; detection limit, strategies for mass and energy selection for MSⁿ; ion axialization, cooling, and remeasurement; and means for guiding externally formed ions into the ion trap. The relation of FT‐ICR MS to other types of Fourier transform spectroscopy and to the Paul (quadrupole) ion trap is described. The article concludes with selected applications, an appendix listing accurate fundamental constants needed for ultrahigh‐precision analysis, and an annotated list of selected reviews and primary source publications that describe in further detail various FT‐ICR MS techniques and applications. © 1998 John Wiley & Sons, Inc., Mass Spec Rev 17, 1–35, 1998