Suppressor of yeast mitochondrial ochre mutations that maps in or near the 15S ribosomal RNA gene of mtDNA.
- 1 March 1982
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 79 (5), 1583-1587
- https://doi.org/10.1073/pnas.79.5.1583
Abstract
A polypeptide chain-terminating mutation in the yeast [Saccharomyces cerevisiae] mitochondrial oxi 1 gene was shown to be an ochre (TAA) mutation by DNA sequence analysis. Mitochondrially inherited revertants of this mutation include 2 types: In the 1st, the ochre codon was changed to a sense codon by further mutation in the oxi 1 gene while, in the 2nd, the ochre codon is still present, indicating the occurrence of an extrageneic ochre suppressor mutation. This mitochondrial ochre suppressor termed MSU1, was cloned in rho- strains of yeast and tested against other oxi 1 mutations. Several additional mutations are also suppressible, and those examined so far are also ochre mutations. MSU1 does not suppress known frameshift or missense mutations at oxi 1. Isoelectric focusing of the gene product (cytochrome oxidase subunit II) from a suppressed-mutant strain indicates that suppression does not involve insertion of charged amino acids. Physical mapping of the mtDNA retained in the MSU1-carrying rho- clones localizes the suppressor mutation to the gene coding the 15S rRNA or a site not more than 300 base pairs from it. No known tRNA genes occur this close to the 15S rRNA gene, and mtDNA from a suppressor-carrying rho- does not hybridize detectably to mitochondrial tRNAs. MSU1 may be an alteration in the 15S rRNA.This publication has 37 references indexed in Scilit:
- Identification of phage SP01 proteins coded by regulatory genes 33 and 34Nature, 1976
- Variant forms of mitochondrial translation products in yeast: evidence for location of determinants on mitochondrial DNA.Proceedings of the National Academy of Sciences, 1976
- Cooperative control of translational fidelity by ribosomal proteins in Escherichia coliMolecular Genetics and Genomics, 1976
- Biogenesis of mitochondria 40. Phenotypic suppression of a mitochondrial mutation by a nuclear gene in Saccharomyces cerevisiae.1975
- High resolution two-dimensional electrophoresis of proteins.1975
- Breakage of yeast: A method for processing multiple samplesAnalytical Biochemistry, 1975
- Suppressors in YeastCurrent Topics in Microbiology and Immunology, 1974
- Alteration of a 30S Ribosomal Protein Accompanying the ram Mutation in Escherichia coliProceedings of the National Academy of Sciences, 1971
- The petite mutation in YeastJournal of Molecular Biology, 1970
- A ribosomal ambiguity mutationJournal of Molecular Biology, 1969