Isolation and Characterization of Anti-H Antibody from Egg Yolk of Immunized Hens
- 1 January 1991
- journal article
- research article
- Published by Taylor & Francis in Immunological Investigations
- Vol. 20 (7), 569-581
- https://doi.org/10.3109/08820139109026238
Abstract
An anti-H antibody was demonstrated to be produced in egg yolk as well as in serum of hens which were immunized with human type O red blood cells. The antibody in egg yolk was isolated with polyethylene glycol and ethanol and was purified by affinity chromatography using immunoadsorbant beads immobilized with H type 2 hapten (Fucai2Ga1β14GlcNAcβ). Hemagglutination reaction of the antibody from egg yolk was inhibited by human saliva samples from secretor types irrespective of their ABO blood types, and by immunoadsorbant beads which contain Fucal2Galβ structures.Keywords
This publication has 11 references indexed in Scilit:
- Production of Antibodies to Canine Distemper Virus in Chicken Eggs for ImmunohistochemistryJournal of Veterinary Medicine, Series B, 1989
- Purification of egg yolk immunoglobulinsJournal of Immunological Methods, 1988
- The Chicken Egg, an Antibody SourceJournal of Veterinary Medicine, Series B, 1986
- Improvements in the isolation of igy from the yolks of eggs laid by immunized hensImmunological Investigations, 1985
- MONOCLONAL ANTI‐TYPE 2 H: AN ANTIBODY DETECTING A PRECURSOR OF THE A AND B BLOOD GROUP ANTIGENSInternational Journal of Immunogenetics, 1982
- Formation of antibodies to prostaglandins in the yolk of chicken eggsBiochemical and Biophysical Research Communications, 1981
- Hen egg yolk as a source of antiviral antibodies in the enzymelinked immunosorbent assay (ELISA): A comparison of two plant virusesJournal of Virological Methods, 1980
- Isolation of Viral IgY Antibodies from Yolks of Immunized HensImmunological Communications, 1980
- On the Specificity of Various Heterologous Anti‐H Hemagglutinins1Vox Sanguinis, 1971
- Ueber natürliche Immunität und ihre Verwerthung für die ImmunisirungstherapieNaunyn-Schmiedebergs Archiv für experimentelle Pathologie und Pharmakologie, 1893