A and B and A1Leb Substances in Glycosphingolipid Fractions of Human Serum1

Abstract
A and B and A1Leb substances were adsorbed onto red cells exposed to glycosphingolipid fractions prepared from the serum of group A and B and A1,Le(a minus b plus) donors. Group O cells exposed to fractions prepared from the serum of group A or B donors were agglutinated by an IgM cross-reacting antibody present in some group O sera. Cells exposed to fractions from A1,Le(a minus b plus) serum were agglutinated by anti-A1Leb. The amount of A substance in the fractions was related to the A subtype (A1 or A2) and to the Lewis and secretor phenotype of the donor. The uptake of blood-group substances from the lipid fractions was inhibited by the addition of whole serum to the fractions.