Callus Induction in Rice, Oryza sativa L.

Abstract

Callus induction in the rice plant, Oryza sativa L. var. Kyoto Asahi was tested by using completely synthetic media. Callus induction depends on the 2, 4-dichlorophenoxyacetic acid (2, 4-D) concentration only, and kinetin is not absolutely essential for callus induction. Callus was also formed on the medium including 2, 4-D and an adenine derivative 1-ethoxy-9-ethyladenine hydroiodide (Y-96). Shikimic acid had no affect on callus induction. The same concentration of indole-3-acetic acid (IAA) as that of 2, 4-D did not induce callus but at 100ppm callus induction occurred. Chromosomal aberration was observed in callus induced from roots and nodes of shoots. Frequency of aneuploid cells was 80% and 57% in calluses from roots and nodes respectively.