A restriction endonuclease from Staphylococcus aureus
Open Access
- 1 November 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 3 (11), 3193-3202
- https://doi.org/10.1093/nar/3.11.3193
Abstract
A specific endonuclease, Sau 3AI, has been partially purified from Staphylococcus aureus strain 3A by DEAE-cellulose chromatography. The enzyme cleaves adenovirus type 5 DNA many times, SV40 DNA eight times but does not cleave double-stranded ϕX174 DNA. It recognizes the sequence and cleaves as indicated by the arrows. Evidence is presented that this enzyme plays a role in the biological restriction-modification system of Staphylococcus aureus strain 3A.
Keywords
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