Further modifications of the auxanographic method for identification of yeasts

Abstract

A modified auxanographic carbohydrate assimilation procedure for the identification of medically important yeasts [Torulopsis glabrata, Candida albicans, C. guilliermondii, C. tropicalis, Cryptococcus [Cr.] albidus vare albidus, Cr. albidus var. diffluens, Cr. neoformans, Cr. unguigattulatus, Rhodotorula rubra, Saccharomyces cerevisiae, Trichosporon cutaneum] is described. This method employs a heavy inoculum of unstarved yeasts, autoclaved yeast assimilation medium, pour plates of shallow depth and commercially available carbohydrate-impregnated disks. The accuracy of this procedure was established in a comparison with the Wickerham broth method.