CONTRIBUTION OF KIDNEY BASEMENT-MEMBRANES AND COLLAGEN TO URINARY-EXCRETION OF HYDROXYLYSYL GLYCOSIDES

Abstract

Glucosyl-galactosyl-hydroxylysine and galactosyl-hydroxylysine are 2 substances present only in collagen and in basement membranes. The kidney contribution to urinary hydroxylysyl glycoside excretion was studied in mongrel dogs. In vitro perfusion of isolated kidneys allows the measurement of hydroxylysyl glycosides independently of the important amount of interstitial collagen present in the whole body. The kidney parenchyma contributes no more than 10% of the glycosylated hydroxylysine excreted in urine. The glomerular basement membrane is not the only renal source of glucosyl-galactosyl-hydroxylysine in urine; tubular basement membrane and interstitial collagen of kidney may contribute to the excretion by the isolated organ.