Modulation of Cell-Surface Antigens of a Murine Neuroblastoma
- 1 January 1974
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 71 (1), 187-191
- https://doi.org/10.1073/pnas.71.1.187
Abstract
Antisera were produced in rabbits to morphologically differentiated cells from the C1300 murine neuroblastoma (i.e., cells in which process formation was induced by maintenance on serum-free medium for 5 days). These antisera reacted more strongly in the complement fixation reaction with such "differentiated" cells than with "undifferentiated" (nonprocess-bearing) neuroblastoma cells. Adsorption of the antisera with undifferentiated cells removed the reactivity to cells without processes, while the reactivity with serum-free cells which possess processes was retained. Indirect immunofluorescence studies confirmed the results obtained by complement fixation and demonstrated that antibodies to the surface antigens of process-bearing cells could be adsorbed by particulate preparations from brain but not liver, spleen, or kidney. This is the first description of neural-associated cell-surface changes that correlate with the morphological differentiation in culture of neuroblastoma cells.Keywords
This publication has 12 references indexed in Scilit:
- Serologically demonstrable cell surface specificities on mouse neuroblastoma C1300.1973
- Glycosphingolipids of Clonal Lines of Mouse Neuroblastoma and Neuroblastoma X L Cell HybridsJournal of Biological Chemistry, 1973
- Surface glycoprotein characteristic of the differentiated state of neuroblastoma C-1300 cellsExperimental Cell Research, 1971
- Induced differentiation of a neuroblastomaDevelopmental Biology, 1971
- Modification of cell surface antigenicity as a function of culture conditionsJournal of Cellular Physiology, 1971
- Immunization schedules for potent rabbit antisera to leukemia L1210.1971
- Induction of Acetylcholinesterase Activity in Mouse Neuroblastoma Tissue Culture CellsNature, 1971
- Regulation of Acetylcholinesterase in Neuroblastoma CellsProceedings of the National Academy of Sciences, 1970
- Regulation of Axon Formation by Clonal Lines of a Neural TumorProceedings of the National Academy of Sciences, 1970
- NEURONAL TUMOR CELLS WITH EXCITABLE MEMBRANES GROWN in vitroProceedings of the National Academy of Sciences, 1969