Transient profiles in ion‐exchange displacement chromatography

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Abstract
A theoretical and experimental study of displacement development in cation‐exchange displacement chromatography is carried out. Simulated displacement chromatograms are generated using a numericl model of displacement chromatography, which employs the steric mass action (SMA) formalism to describe the multicomponent nonlinear adsorption of proteins and polyelectrolytes. Simulated profiles of transient displacement chromatography are compared with experimentla displacement over a ranve of operating conditions, and the model accurately predicts transient displacement behaviour. The success of the model indicates that the SMA formalism is an appropriate tool for prechromatograms indicate that significantly higher throughputs can be achieved by operating these protein displacement systems under nondeveloped conditions. In addition, displacement development proceeds faster at lower salt concentration, indicating that a reduction of the displacement systems. This work sets the stage for the optimization of protien displacement chromatography.