Differences in L-[3H]Glutamate Accumulation and Endogenous L-Glutamate Content in Synaptic Vesicles from Mice Selectively Bred for Differences in Ethanol Sensitivity
- 1 October 1985
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 45 (4), 1294-1297
- https://doi.org/10.1111/j.1471-4159.1985.tb05556.x
Abstract
The ATP-stimulated accumulation of L-[3H]glutamate by whole brain synaptic vesicle preparations from long-sleep and short-sleep mice, lines selectively bred for difference in sleep time response to acute ethanol administration, was examined. L-[3H]Glutamate accumulation in vesicles from short-sleep mice was approximately twice that observed in vesicles from long-sleep mice at three glutamate loading concentrations. The vesicular content of endogenous L-glutamate in crude and enriched vesicle preparations from short-sleep mice was approximately 1.5-fold higher than in vesicles from long-sleep mice. The data suggest that L-glutamate associated with synaptic vesicles may serve a role in glutamate neurosecretion.Keywords
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