The induction of ouabain-resistant mutants by N-methyl-N′-nitro-N-nitrosoguanidine in Chinese hamster cells
- 1 December 1974
- journal article
- research article
- Published by Hindawi Limited in Genetics Research
- Vol. 24 (3), 311-314
- https://doi.org/10.1017/s0016672300015305
Abstract
SUMMARY: Clones of V79 Chinese hamster cells resistant to the steroid compound ouabain have been induced by mutagen treatment with MNNG (= N-methyl-N′-nitro-N-nitrosoguanidine). The frequency of resistant colonies was increased from spontaneous value of 2 × 10−6 to a frequency of 1·2 × 10−3 with MNNG treatment.The highest number of induced mutants appeared when an expression time pf 40 h was allowed between MNNG treatment and addition of ouabain.Keywords
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